Quantitative determination of maleic and succinic acid de - rivatives in the mycelium ofAntrodia cinnamomea

With the successfu1 artificial cultivation of A. cinnaIntroduction moTnea, this fttngus and related products have been widely used as health foods at present. However, quantitative analyAntrodia cinnamomea Chang TT & WN Chou (Chinese sis of the constituents in the products ofA. cinnamomea has name, niu-chang-chih; synonym Antrodia camphorata Wu rarely been conducted. In this paper, we report the cstablishSH et aL) is a medicinal fungus of the family mentofanHPLCmethodforthequantitativedeterrnination Basidiomycetes, which has been used as a folk medicine in offive maleic acid and succinic aeid derivatives. Then using Taiwan. It grows only on the inner heartwood wall of the this HPLC method, the fruiting body and four different lots endemic evergreen Cinnamomum hanehirai Hay. (Lauraceae) of the mycelia ofA, cinnamomea were evaluated. in Taiwan. It is raTe and has not been well cultiyated. The frujting bodies have been used for treating food and drug inMaterials and Methods toxication, diarrhea, abdominal pain, hypertension, itchy skin, and liver cancer.i'5) a few chemical and biological studMaterials. A. cinnamomea mycelium (Ac-Sl 4: Lot ies have been reported hitherto; The pharmacological activinumbers are #C082002m1, #C0921O1 L 1, #C072004-2 ties and the chemical constituents in the crude extract ofA. and #C042503-3, respectively) and its ftuiting body were